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anti-mouse il-22 pe clone 1h8pwsr  (Thermo Fisher)


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    Structured Review

    Thermo Fisher anti-mouse il-22 pe clone 1h8pwsr
    Anti Mouse Il 22 Pe Clone 1h8pwsr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il-22+pe/il+22+cytokine/pmc12115236-24-4-6
    Average 90 stars, based on 1 article reviews
    anti-mouse il-22 pe clone 1h8pwsr - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Staining:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Recombinant:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Cell Isolation:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    SYBR Green Assay:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    cDNA Synthesis:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Emulsion:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Generated:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Software:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Incubation:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Irradiation:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Expressing:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Infection:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Cell Culture:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Real-time Polymerase Chain Reaction:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Isolation:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.

    Fluorescence:

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell markers, cells were fixed and permeabilized via the Foxp3/transcription factor staining buffer set (eBioscience) and stained for IL-22 PE (clone 1H8PWSR; eBioscience).

    Article Title: Epithelium intrinsic vitamin A signaling co-ordinates pathogen clearance in the gut via IL-18
    Article Snippet: Following overnight fixation using the Fixation/Permeabilization solution (eBioscience Foxp3 Staining Buffer Set), cells were permeabilized and stained for cytokines IL-17A AF488 (eBioscience), IL-22 PE (eBioscience) and IFNγ PE (eBioscience).

    Article Title: Role of Common γ-Chain Cytokines in Lung Interleukin-22 Regulation after Acute Exposure to Aspergillus fumigatus
    Article Snippet: After surface staining for appropriate cell 167 markers, cells were fixed and permeablized via the Foxp3/Transcription Factor Staining Buffer Set 168 (eBioscience), and stained for IL-22 PE (clone 1H8PWSR, eBioscience).

    Article Title: Characterization of Mycobacterium tuberculosis -specific Th22 cells and the effect of tuberculosis disease and HIV co-infection
    Article Snippet: Cells were incubated at 4°C for 1 h with the following antibodies: CD19 Pacific Blue (6D5; Biolegend), CD14 Pacific Blue (M5E2; Biolegend), CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), CD45RA BV570 (HI100; Biolegend), CD27 PE-Cy5 (1A4CD27; Beckman Coulter), CCR7 PECF594 (3D12; BD Biosciences), HLA-DR APC-Cy7 (L243; BD Biosciences), IFN-γ Alexa700 (B27; BD Biosciences), IL-17 Alexa 647 (N49-653; BD Biosciences), IL-22 PE (22URTI; e-Bioscience).


    Article Title: Safety, reactogenicity, and immunogenicity of MTBVAC in infants: a phase 2a randomised, double-blind, dose-defining trial in a TB endemic setting
    Article Snippet: Th22 cytokine , IL-22 , PE , 22URTI , eBiosciences , 12-7229-42 , 0.15 , AB_1834463.



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    Journal: Cell host & microbe

    Article Title: Fiber- and acetate-mediated modulation of MHC-II expression on intestinal epithelium protects from Clostridioides difficile infection

    doi: 10.1016/j.chom.2024.12.017

    Figure Lengend Snippet:

    Article Snippet: IL-22 PE Clone 1H8PWSR , Thermo Fisher Scientific , Cat# 12-7221-82, RRID:AB_10597428.

    Techniques: Control, Bacteria, Recombinant, Red Blood Cell Lysis, Cell Recovery, Membrane, Electron Microscopy, Staining, Enzyme-linked Immunosorbent Assay, DNA Purification, Software, Microscopy